Medium 199 has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production and in vitro cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues. Biowest recommends the addition of 10% of Fetal Bovine Serum in the medium. Medium 199 is formulated with either Earle’s salts or Hanks’ salts. These media exist: - with or without L-Glutamine - withor without HEPES - with or without stable Glutamine - with or without Sodium Bicarbonate