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P0134
Ham’s F12 is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat and has proved to be an excellent cloning medium for the culture of myeloma and hybrid cells. Ham’s F12 medium is frequently used with dialysed serum, hormones, selenium and other supplements.
Because Ham’s F12 medium does not contain protein and growth factor, it is recommanded serum supplementation depending the cell type and application.
Its formulation contains no sodium bicarbonate
State
Powder
Storage
+2/+8°C.
Shipping
Room temperature or controlled temperature
Shelf Life
36 months
Sterility
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Product composition details, including formulation components and concentrations. Available as a PDF.
Cell culture
Formulation is without sodium bicarbonate, and, with L-Glutamine and Phenol Red
Glucose concentration : 1.8g/L
To supplement with sodium bicarbonate,
Addition of sodium bicarbonate 7.5% solution (ref: L0680) : 15.7 ml/l
Addition of sodium bicarbonate powder (ref: P2060) : 1.176 g/l
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